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    • AlphaLISA technology and the SciSTREAM add-on for Spotfire provide a quick analysis of various analytical assays

    • Poster presented at SLAS 2014 Conference
    • Authors: Alexandra VamvakidouSeungtaek LeeVanessa Braunstein , Company: PerkinElmer Informatics
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  • Immunoassays are commonly used in the development and characterization of therapeutic proteins, vaccines, and monoclonal antibodies (TAbs). Bioprocess groups developing lot release QC assays are subject to strict regulations, so there is a need for novel techniques that speed drug development, while still meeting regulatory demands. An increasing need for robust, efficient, and sensitive assays to detect and characterize these biologics during various stages of development has led many researchers to AlphaLISA®. PerkinElmer Envision plate reader AlphaLisa data was analyzed and batch-imported with SciSTREAM, an add-on for TIBCO Spotfire. SciSTREAM is a generic data loader to import plate based data from most commercially available instruments. By directly importing raw data from multiple plates, readouts/channels, time points, as well as all the relevant metadata, it’s possible to analyze and compare multi-parametric data from multiple assays and experiments. Plate layout properties can be visually represented in a plate map in Spotfire, enabling scientists to interact with their data, and perform curve fit modeling. We present how AlphaLISA technology and the SciSTREAM add-on for Spotfire provide a versatile workflow of various analytical assays in the modern biotherapeutic development laboratory.

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